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Human LIF Protein, His Tag (MALS verified)

Human LIF Protein, His Tag (MALS verified)
别名: LIF,CDF,DIA,HILDA,MLPLI
储存条件: -20℃ 
Human LIF Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-LIF-H52H3-50ug 50ug 现货 3200.00 3200
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熔点:
密度:
储存条件: -20℃

Human LIF Protein, His Tag (MALS verified)

分子别名(Synonym)

LIF,CDF,DIA,HILDA,MLPLI

表达区间及表达系统(Source)

Human LIF, His Tag s expressed from human 293 cells. It contains AA Ser 23 - Phe 202 

Predicted N-terminus: Ser 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human LIF, His Tag (Cat. No. LIF-H52H3) SDS-PAGE gel

Human LIF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human LIF, His Tag  is more than 85% and the molecular weight of this protein is around 28-42 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LIF, His Tagat 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tagwith a linear range of 1-20 ng/mL (QC tested).

 

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