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Biotinylated Human LAP (TGF-beta 1) (C33S) Protein, Fc,Avitag™ (MALS verified)

Biotinylated Human LAP (TGF-beta 1) (C33S) Protein, Fc,Avitag™ (MALS verified)
别名: LAP (TGF-beta 1),LAP (TGFB1),TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
储存条件: -20℃ 
Biotinylated Human LAP (TGF-beta 1) (C33S) Protein, Fc,Avitag™ (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-LAP-H82F8-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Biotinylated Human LAP (TGF-beta 1) (C33S) Protein, Fc,Avitag™ (MALS verified)

 

分子别名(Synonym)

LAP (TGF-beta 1),LAP (TGFB1),TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

表达区间及表达系统(Source)

Biotinylated Human LAP(C33S), Fc,Avitag is expressed from human 293 cells . It contains AA Leu 30 - Arg 278 .

Predicted N-terminus: Leu 30

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 57.0 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) SDS-PAGE gel

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) MALS images

The purity of Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag is more than 90% and the molecular weight of this protein is around 129-157kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Freeat 5 μg/mL (100 μL/well) can bind Biotinylated Human LAP (TGF-beta 1), Fc,Avitag  with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free t 5 μg/mL (100 μL/well) can bind Biotinylated Human LAP (TGF-beta 1), Fc,Avitag with a linear range of 0.2-13 ng/mL (Routinely tested).

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