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ActiveMax® Human TNFSF11 / RANKL / CD254 Protein, Tag Free (MALS verified)

ActiveMax® Human TNFSF11 / RANKL / CD254 Protein, Tag Free (MALS verified)
别名: RANKL,CD254,TRANCE,OPGL,ODF
储存条件: -20℃ 
ActiveMax® Human TNFSF11 / RANKL / CD254 Protein, Tag Free (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-RAL-H5217-20ug 20ug 现货 1800.00 1800
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熔点:
密度:
储存条件: -20℃

ActiveMax® Human TNFSF11 / RANKL / CD254 Protein, Tag Free (MALS verified)

分子别名(Synonym)

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统(Source)

ActiveMax® Human TNFSF11, Tag Free is expressed from human 293 cells . It contains AA Gly 64 - Asp 245.

Predicted N-terminus: Gly

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 20.5 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

ActiveMax® Human TNFSF11, Tag Free (Cat. No. RAL-H5217) SDS-PAGE gel

ActiveMax® Human TNFSF11, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of ActiveMax® Human TNFSF11, Tag Free is more than 95% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TNFSF11, Tag Free  at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human TNFSF11, Tag Free at 2 μg/mL (100 μL/well) can bind Human Osteoprotegerin, His Tag  with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human TNFSF11, Tag Freeat 2 μg/mL (100 μL/well) can bind Anti-TNFSF11 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

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