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| 别名: | TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human BAFF / TNFSF13B / CD257 Protein, His,Avitag™ (active trimer) (MALS verified)
TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1
Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified)is expressed from human 293 cells . It contains AA Ala 134 - Leu 285
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.2 kDa. The protein migrates as 21-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) is more than 90% in HP-SEC, and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Immobilized Human BCMA, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified)with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Immobilized Human TACI/TNFRSF13B, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA, His Tag at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-BCMA MAb (Mouse IgG1, the antibody was co developed by SCT and ACRObiosystems) and 0.01 μg/mL (100 μL/well) Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) with a half maximal inhibitory concentration (IC50) of 4.877 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Llama IgG2b Fc Tag, low endotoxin : Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) binding reactions. The half maximal inhibitory concentration (IC50) is 1.1323 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag: Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) inding reactions. The half maximal inhibitory concentration (IC50) is 0.7363 μg/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) on SA Chip can bind Human TACI/TNFRSF13B, Fc Tag with an affinity constant of 1.35 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) on SA Chip can bind Human BAFFR, Llama IgG2b Fc Tag, low endotoxin with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
