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Human OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)

Human OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)
别名: 分子别名(Synonym) OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
储存条件: -20℃ 
Human OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-OXL-H52Q8-50ug 50ug 现货 3800.00 3800
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熔点:
密度:
储存条件: -20℃

Human OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统(Source)

Human OX40 Ligand, His Tag (active trimer) is expressed from human 293 cells. It contains AA Gln 51 - Leu 183.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 16.9 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human OX40 Ligand, His Tag (active trimer) (Cat. No. OXL-H52Q8) SDS-PAGE gel

Human OX40 Ligand, His Tag (active trimer) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human OX40 Ligand, His Tag (active trimer) (Cat. No. OXL-H52Q8) MALS images

The purity of Human OX40 Ligand, His Tag (active trimer) is more than 95% in HP-SEC, and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human OX40 Ligand, His Tag (active trimer) at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag with a linear range of 1-8 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human OX40 Ligand, His Tag (active trimer) (MALS verified) at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Fc,Avitagwith a linear range of 10-78 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human OX40, Avitag,His Tag  on SA Biosensor, can bind Human OX40 Ligand, His Tag (active trimer)  with an affinity constant of 0.127 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human OX40 Ligand, His Tag (active trimer)on AR2G Biosensor, can bind Human OX40, His Tag with an affinity constant of 0.15 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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