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| 别名: | CD70,CD27LG,TNFSF7,TNFSF7G,CD27L | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human CD27 Ligand / CD70 Protein, His,Flag Tag (active trimer) (MALS verified)
CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) is expressed from human 293 cells . It contains AA Ser 52 - Pro 193 trimer Design.
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) is more than 90% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Immobilized Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) at 2 μg/mL (100 μL/well) can bind Human CD27, Fc Tag with a linear range of 2-125 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) : Biotinylated Human CD27, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.42856 μg/mL (Routinely tested).
