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Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™

Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™
别名: IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC
储存条件: -20℃ 
Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILG-H85E8-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™

分子别名(Synonym)

IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC

表达区间及表达系统(Source)

Biotinylated Human IL-2 R gamma, His,Avitag  is expressed from Baculovirus-Insect cells. It contains AA Leu 23 - Asn 254 .

Predicted N-terminus: Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) SDS-PAGE gel

Biotinylated Human IL-2 R gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Biotinylated Human IL-2 R gamma, His,Avitag inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 4.08-5.90 µg/mL in the presence of 10 µg/mL of human IL-2 R beta and 50 ng/mL of human IL-2 (Routinely tested).

分子别名(Synonym)

IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC

表达区间及表达系统(Source)

Biotinylated Human IL-2 R gamma, His,Avitag (ILG-H85E8) is expressed from Baculovirus-Insect cells. It contains AA Leu 23 - Asn 254 

Predicted N-terminus: Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) SDS-PAGE gel

Biotinylated Human IL-2 R gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Biotinylated Human IL-2 R gamma, His,Avitag  inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 4.08-5.90 µg/mL in the presence of 10 µg/mL of human IL-2 R beta and 50 ng/mL of human IL-2 (Routinely tested).

 

 

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