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Human IL-17 RA / CD217 Protein, His Tag

Human IL-17 RA / CD217 Protein, His Tag
别名: CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R
储存条件: -20℃ 
Human IL-17 RA / CD217 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILA-H5222-200ug 200ug 现货 2800.00 2800
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熔点:
密度:
储存条件: -20℃

 

Human IL-17 RA / CD217 Protein, His Tag

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Human IL-17 RA, His Tag is expressed from human 293 cells . It contains AA Leu 33 - Trp 320.

Predicted N-terminus: Leu 33

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 34.3 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-17 RA, His Tag (Cat. No. ILA-H5222) SDS-PAGE gel

Human IL-17 RA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL17A, Tag Free  at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL17A, His,Avitag on SA Biosensor, can bind Human IL-17 RA, His Tag with an affinity constant of 18.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Biotinylated Human IL-17A&IL-17F, Avitag,His Tag  on SA Biosensor, can bind Human IL-17 RA, His Tag with an affinity constant of 352 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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