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储存条件: | -20℃ |
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储存条件: | -20℃ |
GMP Human IL-15
Designed under ISO 9001:2015 and ISO 13485:2016
Manufactured and QC tested under a GMP compliance factory
Animal-Free materials
Batch-to-batch consistency
Stringent quality control tests
GMP Human IL-15 is expressed from E.coli. It contains AA Asn 49 - Ser 162 .
Predicted N-terminus: Met
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. The protein migrates as 12-13 kDa under reducing (R) condition (SDS-PAGE).
Less than 10 EU/mg by the LAL method.
<0.5ng/ug of protein tested by ELISA.
<0.02ng/μg of protein tested by ELISA.
The sterility testing was performed by Membrane Filtration Method described in CP<1101>.
Negative
Negative
≥95% as determined by SDS-PAGE.
25mM His, pH6.2 with trehalose as protectant.
Please see Certificate of Analysis for specific instructions.
Shipping at ambient temperature. Upon receipt, store it immediately at -20°C or lower for long term storage.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state from date of receipt;
-70°C for 12 months under sterile conditions after reconstitution.
GMP Human IL-15 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
GMP Human IL-15 stimulates proliferation of CTLL-2 cells. The EC50 for this effect is 1.004 ng/mL, corresponding to a specific activity of > 0.8ⅹ10^7 IU/mg, which is calibrated against human IL- 15 WHO International Standard (QC tested).
Protocol
The Cell based assay shows that GMP Human IL-15 is stable at 25°C for 72 hours and after freezing and thawing 3 times.