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GMP Human IL-15

GMP Human IL-15
别名:
储存条件: -20℃ 
GMP Human IL-15
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GMP-L15H13-50ug 50ug 现货 5000.00 5000
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熔点:
密度:
储存条件: -20℃

 

GMP Human IL-15

  1. Designed under ISO 9001:2015 and ISO 13485:2016

  2. Manufactured and QC tested under a GMP compliance factory

  3. Animal-Free materials

  4. Batch-to-batch consistency

  5. Stringent quality control tests

表达区间及表达系统(Source)

GMP Human IL-15 is expressed from E.coli. It contains AA Asn 49 - Ser 162 .

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 12.8 kDa. The protein migrates as 12-13 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 10 EU/mg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5ng/ug of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02ng/μg of protein tested by ELISA.

无菌(Sterility)

The sterility testing was performed by Membrane Filtration Method described in CP<1101>.

支原体(Mycoplasma)

Negative

外源病毒(In vitro virus assay)

Negative

纯度(Purity)

≥95% as determined by SDS-PAGE.

制剂(Formulation)

25mM His, pH6.2 with trehalose as protectant.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

 

存储(Storage)

Shipping at ambient temperature. Upon receipt, store it immediately at -20°C or lower for long term storage.

 

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state from date of receipt;

  2. -70°C for 12 months under sterile conditions after reconstitution.

电泳(SDS-PAGE)

GMP Human IL-15 (Cat. No. GMP-L15H13) SDS-PAGE gel

GMP Human IL-15 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

GMP Human IL-15  stimulates proliferation of CTLL-2 cells. The EC50 for this effect is 1.004 ng/mL, corresponding to a specific activity of > 0.8ⅹ10^7 IU/mg, which is calibrated against human IL- 15 WHO International Standard  (QC tested).

Protocol

稳定性(Stability)

Biotinylated Human Stability_ELISA

The Cell based assay shows that GMP Human IL-15 is stable at 25°C for 72 hours and after freezing and thawing 3 times.

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