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| 别名: | IL-15,Interleukin-15,MGC9721 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-15 Protein, Tag Free (SPR verified)
IL-15,Interleukin-15,MGC9721
Human IL-15, Tag Free is expressed from E.coli cells. It contains AA Asn 49 - Ser 162.
Predicted N-terminus: Met
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-15, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-15, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tagwith a linear range of 0.6-39 ng/mL (QC tested).
Protocol
Human IL-2 R beta, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Loaded Human IL-2 R beta, Fc Tag (SPR verified)n Protein A Biosensor, can bind Human IL-15, Tag Freewith an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human IL-15, Tag Free stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/mL (Routinely tested).
