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Cynomolgus IL-33 Protein, His Tag (MALS verified)

Cynomolgus IL-33 Protein, His Tag (MALS verified)
别名: IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2
储存条件: -20℃ 
Cynomolgus IL-33 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL3-C5245-5 50ug 现货 0.00
ZY-IL3-C5245-50ug 50ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Cynomolgus IL-33 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

表达区间及表达系统(Source)

Cynomolgus IL-33, His Tag  is expressed from human 293 cells. It contains AA His 109 - Ile 270

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Cynomolgus IL-33, His Tag (Cat. No. IL3-C5245) SDS-PAGE gel

Cynomolgus IL-33, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Cynomolgus IL-33, His Tag  is more than 90% and the molecular weight of this protein is around 19-29 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus IL-33, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-1RL1, Fc Tagwith a linear range of 2-78 ng/mL (QC tested).

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