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| 别名: | IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)
IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG
Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is expressed from human 293 cells. It contains AA Ala 27 - Glu 240 (IL-2RB) & Trp 23 - Ala 263 (IL-2RG).
Request for sequence
Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.4 kDa (IL-2RB) and 35.0 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 50-60 kDa and 62-66 kDa respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tagis more than 85% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.
Report
Loaded Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on NTA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 1.57 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
