Anti-ARF1 antibody [E9-D8]

Anti-ARF1 antibody [E9-D8]

• 概述

• 产品描述ADP-ribosylation factors (ARFs), are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin, and constitute one family of the RAS superfamily. ARFs are essential and ubiquitous in eukaryotes, as they are involved in vesicular transport and functioning via phospholipase D activation. ARF proteins play a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. ARF1 is regulated by the binding and hydrolysis of GTP. Coatomer, or COPI, is a heptameric protein recruited to membranes by ARF1. Research demonstrates that guanine nucleotide exchange-activated ARF1, when located at the Golgi membrane, recruits and binds cytoplasmic COPI to the membranes.
• 产品名称Anti-ARF1 antibody [E9-D8]
• 分子量21 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Golgi apparatus. Cytoplasm
• 其它名称
  • ADP Ribosylation Factor 1 antibody
  • ADP-ribosylation factor 1 antibody
  • ARF 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200 IHC-P:1:50-1:200
  FC: 1:50-1:200

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  Fig1: Western blot analysis of ARF1 on human ARF1 recombinant protein using anti-ARF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ARF1 on HEK293 (1) and ARF1-hIgGFc transfected HEK293 (2) cell lysate using anti-ARF1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining ARF1 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining ARF1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with ARF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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