Anti-ICAM4 antibody

Anti-ICAM4 antibody

• 概述

• 产品描述ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
• 产品名称Anti-ICAM4 antibody
• 分子量27 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ICAM4 85-180/271
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted.
• 其它名称CD242
  CD242 antigen
  ICAM-4
  Icam4
  ICAM4_HUMAN
  Intercellular adhesion molecule 4
  Landsteiner Wiener blood group glycoprotein
  Landsteiner-Wiener blood group glycoprotein
  LW
  LW blood group protein.
  more

• 应用

  WB:1:500-2000
  FC:1ug/test

•  

  

  Fig1: Sample: K562 Cell (Human) Lysate at 40 ug
  Primary: Anti-ICAM4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 45 kD

   

  

  Fig2: Sample: Bone (Mouse) Lysate at 40 ug
  Primary: Anti-ICAM4at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 45 kD

   

  

  Fig3: Sample: placenta(Mouse) Lysate at 40 ug
  Primary: Anti-ICAM4at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 45 kD

   

  

  Fig4: Blank control: Hela.
  Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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