Anti-KLHL13 antibody [B12-D1]

Anti-KLHL13 antibody [B12-D1]

• 概述

• 产品描述Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

• 产品名称Anti-KLHL13 antibody [B12-D1]

• 分子量74 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cytosol.

   

• 其它名称

  more

  • BKLHD2 antibody

  • BTB and kelch domain containing 2 antibody

  • BTB and kelch domain containing 2 KIAA1309 antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

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  Fig1: Western blot analysis of KLHL13 on Hela(1) and MCF-7 (2) cell lysate using anti-KLHL13 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining KLHL13 (green) and Actin filaments (red) in NTERA-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining KLHL13 (green) and Actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-KLHL13 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of 3T3/L1 cells with KLHL13 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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