Anti-HLA Class 1 ABC antibody [11C3]

Anti-HLA Class 1 ABC antibody [11C3]

• 概述

• 产品描述HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
• 产品名称Anti-HLA Class 1 ABC antibody [11C3]
• 分子量40 kDa

• 种属反应性Human

• 验证应用WB,ICC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein within Human HLA Class 1 ABC aa 120-310.

• 偶联Non-conjugated

• Anti-HLA Class 1 ABC antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein affinity purified.

• 亚细胞定位Cell Membrane.

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• 其它名称

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  • Antigen presenting molecule antibody

  • HLA class I histocompatibility antigen, A 1 alpha chain antibody

  • HLAA antibody

• 应用

  WB:1:500-1:2000
  ICC:1:50-1:200

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  Fig1: Western blot analysis of HLA Class 1 ABC on Hela lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801).

   

  

  Fig2: Immunocytochemistry staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ki67 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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