Anti-SMARCA1 antibody [B9-H7]

Anti-SMARCA1 antibody [B9-H7]

• 概述

• 产品描述Energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. Both complexes facilitate the perturbation of chromatin structure in an ATP-dependent manner. Potentiates neurite outgrowth. May be involved in brain development by regulating En-1 and En-2 expression. May be involved in the development of luteal cells.
• 产品名称Anti-SMARCA1 antibody [B9-H7]
• 分子量123 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • ATP dependent helicase SMARCA1 antibody
  • global transcription activator homologous sequence antibody
  • ISWI antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of SMARCA1 on human SMARCA1 recombinant protein using anti-SMARCA1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of SMARCA1 on HEK293 (1) and SMARCA1-hIgGFc transfected HEK293 (2) cell lysate using anti-SMARCA1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of SMARCA1 on different cell lysate using anti-SMARCA1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: PANC-1
  Lane 2: HEK293
  Lane 3: SW620
  Lane 4: HT-29
  Lane 5: SH-SY5Y
  Lane 6: SK-OV-3

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-SMARCA1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-SMARCA1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of NIH/3T3 cells with SMARCA1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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