Anti-HAS2 antibody [C4-E7]

Anti-HAS2 antibody [C4-E7]

• 概述

• 产品描述HAS1, HAS2 and HAS3 are HA Synthase proteins that synthesize HA (Hyaluronan or hyaluronic acid). The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by β-1,3 and β-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesise high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli suggesting distinct functional roles of the three proteins. HAS2 mRNA shows predominant expression in chondrocytes and cartilage. The human HAS2 gene maps to chromosome 8q24.12.

• 产品名称Anti-HAS2 antibody [C4-E7]

• 分子量64 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Membrane.

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• ​其它名称

  more

  • HA synthase 2 antibody

  • has2 antibody

  • HAS2_HUMAN antibody

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of HAS2 on human HAS2 recombinant protein using anti-HAS2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of HAS2 on NTERA-2 (1) and HEK293 (2) cell lysate using anti-HAS2 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining HAS2 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HAS2 antibody. Counter stained with hematoxylin.

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