Anti-PIWIL4 antibody [B11-G9]

Anti-PIWIL4 antibody [B11-G9]

• 概述

• 产品描述PIWIL4 (PIWI-like protein 4), also known as HIWI2 is a 852 amino acid protein that belongs to the argonaute family. PIWIL4 contains one PAZ domain and one PIWI domain and is essential for the maintenance of germline stem cells. PIWIL4 is a cytoplasmic protein that is expressed in adult testis. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. PIWIL4-null mice are of normal size and weight and have the expected life span. Homozygous PIWIL4-null females are fertile and have no obvious defects. However, PIWIL4-deficient males are infertile and show a meiotic progression defect in early prophase of meiosis I and progressive loss of germ cells with age. Mutant males show elevated expression of LINE-1 and intracisternal A particle (IAP) element transcripts in germ cell lineages. The gene encoding PIWIL4 maps to human chromosome 11.
• 产品名称Anti-PIWIL4 antibody [B11-G9]
• 分子量97 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm.
• 其它名称
  • DKFZp686P01248 antibody
  • FLJ36156 antibody
  • HILI 2 antibody
  more

• 应用

  WB: 1:500
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PIWIL4 on human PIWIL4 recombinant protein using anti-PIWIL4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PIWIL4 on HEK293 (1) and PIWIL4-hIgGFc transfected HEK293 (2) cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PIWIL4 on different cell lysate using anti-PIWIL4 antibody at 1/1,000 dilution.
  Positive control:
  Line1:PC-3 Line2:PANC-1

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PIWIL4 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with PIWIL4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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