Anti-KEAP1 antibody [B10-G]

Anti-KEAP1 antibody [B10-G]

• 概述

• 产品描述Keap1 (kelch-like ECH-associated protein 1, INrf2, KLHL19) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates Nrf2 (NF-E2-related factor 2). Steady state levels of proteins are under the influence of the ubiquitin pathway, which consists of ubiquitin activation (E1), conjugation (E2) and ligation (E3). Keap1 assembles into an E3 ubiquitin ligase complex with Cul3 and Rbx1 and targets lysine residues in the N-terminal Neh2 domain of Nrf2 for ubiquitin conjugation. The Keap1-Nrf2 system mediates cytoprotective gene expression in response to oxidative and/or electrophilic stresses. Keap1 constitutively suppresses Nrf2 activity under unstressed conditions, oxidants or electrophiles provoke the repression of Keap1 activity, inducing Nrf2 activation. Cys 273 and Cys 288 residues of Keap1 are required for suppressing Nrf2 nuclear accumulation. Keap1 sequesters Nrf2 in the cytoplasm through an active Crm1/exportin-dependent nuclear export mechanism.
• 产品名称Anti-KEAP1 antibody [B10-G]
• 分子量70 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
• 其它名称
  • Cytosolic inhibitor of Nrf2 antibody
  • INrf 2 antibody
  • INrf2 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of KEAP1 on human KEAP1 recombinant protein using anti-KEAP1 antibody at 1/1,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-KEAP1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-KEAP1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with KEAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！