Anti-CD40 antibody
-
概述
- 产品描述Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. A receptor ligand pair central to the trans-mission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif.
- 产品名称Anti-CD40 antibody
- 分子量Predicted band size 31kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human CD40 aa 10-250.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Plasma membrane. Secreted.
- 其它名称AI326936 antibody
B cell associated molecule CD40 antibody
B cell surface antigen CD40 antibody
B cell-associated molecule antibody
B-cell surface antigen CD40 antibody
Bp50 antibody
CD 40 antibody
CD40 antibody
CD40 antigen (TNF receptor superfamily member 5) antibody
CD40 antigen antibody
CD40 molecule antibody
CD40 molecule, TNF receptor superfamily member 5 antibody
CD40 protein antibody
CD40 type II isoform antibody
CD40L receptor antibody
CDw40 antibody
GP39 antibody
HIGM1 antibody
IGM antibody
IMD3 antibody
MGC9013 antibody
Nerve growth factor receptor related B lymphocyte activation molecule antibody
OTTHUMP00000031699 antibody
OTTHUMP00000031700 antibody
p50 antibody
T-BAM antibody
TBAM antibody
TNF receptor superfamily member 5 antibody
TNFRSF5 antibody
TNR5_HUMAN antibody
TRAP antibody
Tumor necrosis factor receptor superfamily , member 5 antibody
Tumor necrosis factor receptor superfamily member 5 antibody
Tumor necrosis factor receptor superfamily member 5 precursor antibody
Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
more
-
应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50 -1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.
Fig2: ICC staining CD40 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD40 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD40 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD40 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!