Anti-LEF1 antibody [B11-A4]

Anti-LEF1 antibody [B11-A4]

• 概述

• 产品描述The TCF (T cell factor) family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. The TCF (T cell factor) proteins, which are required during development, include TCF-1, which is essential for lymphoid cell development, and TCF-3 and TCF-4, which are implicated in neuronal development and LEF (leukemia enhancer factor).The Wnt mediated signaling pathway induces cytosolic β-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β-catenin and, in turn, increases the degradation of cytosolic β-catenin to, thereby, inhibit the transcriptional activity of the TCF proteins. Mutations in the APC gene, which are commonly observed in colorectal carcinomas, disrupt this regulatory pathway and correlate to an accumulation of β-catenin and the increased activation of the TCF target genes.
• 产品名称Anti-LEF1 antibody [B11-A4]
• 分子量44 kDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
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• 其它名称
  • DKFZp586H0919 antibody
  • FLJ46390 antibody
  • LEF 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of LEF1 on human LEF1 recombinant protein using anti-LEF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of LEF1 on HEK293 (1) and LEF1-hIgGFc transfected HEK293 (2) cell lysate using anti-LEF1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of LEF1 on different cell lysate using anti-LEF1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: HepG2
  Lane 3: MOLT4

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-LEF1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-LEF1 antibody. Counter stained with hematoxylin.

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