Anti-Rab5a antibody [C11-H5]

Anti-Rab5a antibody [C11-H5]

• 概述

• 产品描述The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, RAP, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves, at each stage, the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
• 产品名称Anti-Rab5a antibody [C11-H5]
• 分子量24 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • RAB 5 antibody
  • RAB 5A antibody
  • RAB5A antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Rab5a on human Rab5a recombinant protein using anti-Rab5a antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Rab5a on HEK293 (1) and Rab5a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab5 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Rab5a on Hela (1) and K562 (2) cell lysate using anti-Rab5a antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining Rab5a (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Rab5a antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human duodenum tissue using anti-Rab5a antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with Rab5a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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