Anti-XRN2 antibody

Anti-XRN2 antibody

• 概述

• 产品描述Degradation of mRNA is a critical aspect of gene expression that occurs via the exoribonuclease. Exoribonuclease 2 (XRN2) is the human homologue of the Saccharomyces cerevisiae RAT1, which functions as a nuclear 5' to 3' exoribonuclease and is essential for mRNA turnover and cell viability. XRN2 also processes rRNAs and small nucleolar RNAs (snoRNAs) in the nucleus. XRN2 moves along with RNA polymerase II and gains access to the nascent RNA transcript after the endonucleolytic cleavage at the poly(A) site or at a second cotranscriptional cleavage site (CoTC). CoTC is an autocatalytic RNA structure that undergoes rapid self-cleavage and acts as a precursor to termination by presenting a free RNA 5' end to be recognized by XRN2. XRN2 then travels in a 5'-3' direction like a guided torpedo and facilitates the dissociation of the RNA polymerase elongation complex.
• 产品名称Anti-XRN2 antibody
• 分子量109 kDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Nucleolus.
• 其它名称
  • 5' 3' exoribonuclease 2 antibody
  • DHM1 like protein antibody
  • DHP protein antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1:500

•  

  

  Fig1: Western blot analysis of XRN2 on human XRN2 recombinant protein using anti-XRN2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of XRN2 on HEK293 (1) and XRN2-hIgGFc transfected HEK293 (2) cell lysate using anti-XRN2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of XRN2 on different cell lysate using anti-XRN2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Raw264.7
  Lane 2: HEK293
  Lane 3: NTERA-2
  Lane 4: LNcap
  Lane 5: HepG2
  Lane 6: HEK293
  Lane 7: Hela

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XRN2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- XRN2 antibody. Counter stained with hematoxylin.

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