Anti-FAM62B antibody [C4-D0]

Anti-FAM62B antibody [C4-D0]

• 概述

• 产品描述Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex. Promotes the localization of SACM1L at endoplasmic reticulum-plasma membrane contact sites (EPCS).
• 产品名称Anti-FAM62B antibody [C4-D0]
• 分子量102 kDa
• 种属反应性Human
• 验证应用WB,IHC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human FAM62B aa 300-550.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G affinity purified.
• 亚细胞定位Cell membrane, Endoplasmic reticulum, Membrane.
• 其它名称
  • Chr2 synaptotagmin antibody
  • CHR2SYT antibody
  • E Syt2 antibody
  more

• 应用

  WB: 1:500
  IHC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of FAM62B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Siha cell lysate
  Lane 2: THP-1 cell lysate

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FAM62B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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