Anti-SK2 antibody [E1-C5]

Anti-SK2 antibody [E1-C5]

• 概述

• 产品描述This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• 产品名称Anti-SK2 antibody [E1-C5]
• 分子量69 kDa
• 种属反应性Human
• 验证应用ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol
• 其它名称
  • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
  • 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
  • ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
  more

• 应用

  ICC: 1:200-1:1000
  IHC-P: 1:200-1:1000
  FC: 1:50-1:100

•  

  

  Fig1: ICC staining SPHK2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with SPHK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！