Anti-UBE2C antibody [5D-3F]

Anti-UBE2C antibody [5D-3F]

• 概述

• 产品描述The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.
• 产品名称Anti-UBE2C antibody [5D-3F]
• 分子量20 kDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, cytosol, nucleoplasm , plasma membrane.
• 其它名称
  • Cyclin selective ubiquitin carrier protein antibody
  • dJ447F3.2 antibody
  • Mitotic specific ubiquitin conjugating enzyme antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of UBE2C on human UBE2C recombinant protein using anti-UBE2C antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of UBE2C on HEK293 (1) and UBE2C-hIgGFc transfected HEK293 (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of UBE2C on Hela (1) and Raji (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.

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