Anti-SIRT7 antibody [G10-E2]

Anti-SIRT7 antibody [G10-E2]

• 概述

• 产品描述Sirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal DNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer. Two isoforms exist due to alternative splicing events.
• 产品名称Anti-SIRT7 antibody [G10-E2]
• 分子量45 kDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus
• 其它名称
  • NAD dependent deacetylase sirtuin 7 antibody
  • NAD dependent protein deacetylase sirtuin 7 antibody
  • NAD-dependent protein deacetylase sirtuin-7 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of SIRT7 on human SIRT7 recombinant protein using anti-SIRT7 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of SIRT7 on HEK293 (1) and SIRT7-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT7 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SIRT7 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-SIRT7 antibody. Counter stained with hematoxylin.

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