Anti-PPP1CB antibody [A7-C7]

Anti-PPP1CB antibody [A7-C7]

• 概述

• 产品描述In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.
• 产品名称Anti-PPP1CB antibody [A7-C7]
• 分子量37 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus
• 其它名称
  • MGC3672 antibody
  • PP 1B antibody
  • PP-1B antibody
  more

• 应用

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PPP1CB on human PPP1CB recombinant protein using anti-PPP1CB antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PPP1CB on HEK293 (1) and PPP1CB-hIgGFc transfected HEK293 (2) cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PPP1CB on different cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.
  Positive control: Line1: Jurkat Line2: A431 Line3: Hela Line4: HepG2 Line5: HEK293 Line6: MCF-7

   

  

  Fig4: ICC staining PPP1CB (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with PPP1CB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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