Anti-CALB2 antibody [H1-F5]

Anti-CALB2 antibody [H1-F5]

• 概述

• 产品描述Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.
• 产品名称Anti-CALB2 antibody [H1-F5]
• 分子量32 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol
• 其它名称
  • 29 kDa calbindin antibody
  • CAB 29 antibody
  • CAB29 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Calretinin on human Calretinin recombinant protein using anti- Calretinin antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Calretinin on HEK293 (1) and Calretinin -hIgGFc transfected HEK293 (2) cell lysate using anti- Calretinin antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- Calretinin antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of A549 cells with Calretinin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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