Anti-CHRNA7 antibody [G1-F10]

Anti-CHRNA7 antibody [G1-F10]

• 概述

• 产品描述Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα7, also known as NACHRA7, CHRNA7-2 or CHRNA7, is a 502 amino acid multi-pass membrane protein existing as a homopentamer and interacts with RIC-3, a nicotinic acetylcholine receptor (nAChR)-associated protein.
• 产品名称Anti-CHRNA7 antibody [G1-F10]
• 分子量56 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell junction, synapse, postsynaptic cell membrane. Cell membrane
  
• 其它名称
  • 7nAChR antibody
  • a7 nicotinic acetylcholine receptor antibody
  • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of CHRNA7 on human CHRNA7 recombinant protein using anti-CHRNA7 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CHRNA7 on HEK293 (1) and CHRNA7-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA7 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CHRNA7 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CHRNA7 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of SH-SY5Y cells with CHRNA7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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