Anti-KBTBD8 antibody [6G-8A]

Anti-KBTBD8 antibody [6G-8A]

• 概述

• 产品描述Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification (PubMed:26399832). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
• 产品名称Anti-KBTBD8 antibody [6G-8A]
• 分子量69 kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, cytoskeleton
• 其它名称
  • AU040152 antibody
  • FLJ57592 antibody
  • KBTB8_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of KBTBD8 on human KBTBD8 recombinant protein using anti-KBTBD8 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of KBTBD8 on HEK293 (1) and KBTBD8-hIgGFc transfected HEK293 (2) cell lysate using anti-KBTBD8 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of KBTBD8 on different cell lysate using anti-KBTBD8 antibody at 1/1,000 dilution.
  Positive control: Line1: A431 Line2: Jurkat Line3: Hela Line4: K562 Line5: Hek293

   

  

  Fig4: Flow cytometric analysis of A431 cells with KBTBD8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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