Anti-PI 3-kinase p85α/γ antibody

Anti-PI 3-kinase p85α/γ  antibody

• 概述

• 产品描述Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• 产品名称Anti-PI 3-kinase p85α/γ antibody
• 分子量54/83 kDa
• 种属反应性Human, Mouse, Rat, Monkey
• 验证应用WB, IHC-P, IF
• 抗体类型兔多抗
• 免疫原The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma. AA range:436-485
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• 亚型IgG
• 纯化方式Immunogen affinity purified.
• 其它名称
  • PIK3R1 antibody; GRB1 antibody; Phosphatidylinositol 3-kinase regulatory subunit alpha antibody; PI3-kinase regulatory subunit alpha antibody; PI3K regulatory subunit alpha antibody; PtdIns-3-kinase regulatory subunit alpha antibody; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph antibody;

• 应用

  WB: 1:500-1:2000
  IHC-P: 1:100-1:300
  IF: 1:100-1:500

•  

  

  Fig1: Immunofluorescence analysis of human-lung tissue. 1,PI 3-kinase p85α/γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig2: Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PI 3-kinase p85α/γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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