Anti-HSF1 antibody [4D-5F]

Anti-HSF1 antibody [4D-5F]

• 概述

• 产品描述Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway
• 产品名称Anti-HSF1 antibody [4D-5F]
• 分子量57 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, cytoplasm.
• 其它名称
  • Heat shock factor 1 antibody
  • Heat shock factor protein 1 antibody
  • Heat shock transcription factor 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of HSF1 on human HSF1 recombinant protein using anti-HSF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of HSF1 on HEK293 (1) and HSF1-hIgGFc transfected HEK293 (2) cell lysate using anti-HSF1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining HSF1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-HSF1 antibody. Counter stained with hematoxylin.

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