Anti-DNase gamma antibody

Anti-DNase gamma antibody

• 概述

• 产品描述Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units.
• 产品名称Anti-DNase gamma antibody
• 分子量36 KDa
• 种属反应性Human,Mouse,Rat,Pig,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human DNase gamma 151-250/305
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus.
• 数据链接SwissProt: Q13609 Human
  
• 其它名称Deoxyribonuclease gamma
  Deoxyribonuclease I like 3
  Deoxyribonuclease I like III
  Deoxyribonuclease I-like 3
  DHP2
  DNAS1L3
  DNase gamma
  DNase I homolog protein 2
  DNase I homolog protein DHP2
  DNase I like 3
  DNase I-like 3
  DNASE1L3
  DNSL3_HUMAN
  Liver and spleen DNase
  LS DNase
  LS-DNase
  LSD.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-DNase gamma Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  .

   

  

  Fig2: Sample:
  Liver (Mouse) Lysate at 40 ug
  Spleen (Mouse) Lysate at 40 ug
  Primary: Anti-DNase gammaat 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 36 kD
  Observed band size: 32/36 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！