Anti-EIF2AK2 antibody [3A-H10]

Anti-EIF2AK2 antibody [3A-H10]

• 概述

• 产品描述Interferon-inducible RNA-dependent protein serine/threonine kinase, PKR, is variously designated in earlier literature as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68, eIF-2α protein kinase or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA-mediated autophosphorylation.
• 产品名称Anti-EIF2AK2 antibody [3A-H10]
• 分子量62 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus. Cytoplasm ? perinuclear region
• 其它名称
  • Double stranded RNA activated protein kinase; antibody
  • E2AK2_HUMAN antibody
  • eIF-2A protein kinase 2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1: 500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of EIF2AK2 on human EIF2AK2 recombinant protein using anti- EIF2AK2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of EIF2AK2 on HEK293 (1) and EIF2AK2-hIgGFc transfected HEK293 (2) cell lysateusing using anti-FTL antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of EIF2AK2 on cells lysates using anti- EIF2AK2 antibody at 1/1,000 dilution.
  Positive control: Line1: A431 Line2: THP-1 Line3: MCF-7 Line4: PC-12

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- EIF2AK2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissuesusing anti-EIF2AK2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with EIF2AK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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