Anti-SARS-CoV-2 Nucleocapsid protein antibody [A5B10]

Anti-SARS-CoV-2 Nucleocapsid protein antibody [A5B10]

• 概述

• 产品描述The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• 产品名称Anti-SARS-CoV-2 Nucleocapsid protein antibody [A5B10]
• 分子量46 kDa
• 种属反应性SARS-CoV-2
• 验证应用WB,ELISA
• 抗体类型小鼠单抗
• 免疫原Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH 7.4).
• 亚型IgG1
• 纯化方式Protein G affinity purified.
• 亚细胞定位Virion.
• 其它名称
  • 2019-nCoV nucleocapsid protein antibody
  • N antibody
  • NC antibody
  more

• 应用

  WB: 0.5-2ug/ml
  ELISA: 10ng/ml

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  Fig1: The binding activity of HA600007 with Recombinant SARS-CoV-2 Nucleocapsid protein.
  Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600007) for 1 hour at 25℃. The EC50 of HA600007 is 11.82 ng/ml.

   

  

  Fig2: This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Cat: # HA600007). In a sandwich ELISA, it can be used as Capture antibody when paired with (Cat: # HA600005).

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