Anti-INCENP antibody [G9-D2]

Anti-INCENP antibody [G9-D2]

• 概述

• 产品描述A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex.
• 产品名称Anti-INCENP antibody [G9-D2]
• 分子量105 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoskeleton. Nucleus.
• 其它名称
  • binds and activates aurora B and C in vivo and in vitro antibody
  • Chromosomal passenger protein antibody
  • INCE_HUMAN antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; re

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