Anti-TWF1 antibody [A2-C11]

Anti-TWF1 antibody [A2-C11]

• 概述

• 产品描述Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein. Phosphatidylinositol (4,5)-bisphosphate inhibits the activity of Twinfilin-1, while two small GTPases, namely Rac1 and Cdc42, induce the redistribution of Twinfilin-1 to membrane ruffles and cell-cell contacts, respectively.
• 产品名称Anti-TWF1 antibody [A2-C11]
• 分子量40 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Cytoskeleton.
• 其它名称
  • A6 antibody
  • A6 protein tyrosine kinase antibody
  • MGC105817 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:100
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of TWF1 on human TWF1 recombinant protein using anti-TWF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of TWF1 on HEK293 (1) and TWF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWF1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining TWF1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-TWF1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with TWF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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