Anti-ApoB antibody [B1-G6]

Anti-ApoB antibody [B1-G6]

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• 概述

• 产品描述Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB-100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.
• 产品名称Anti-ApoB antibody [B1-G6]
• 分子量516 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Extracellular region. Secreted.
• 其它名称
  • apo b-100 antibody
  • APOB antibody
  • apoB-48R antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of ApoB on human ApoB recombinant protein using anti-ApoB antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining ApoB (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Flow cytometric analysis of PBMC cells with ApoB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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