Anti-CD105 antibody [H4-A9]

Anti-CD105 antibody [H4-A9]

• 概述

• 产品描述Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant disorder characterized by vascular abnormalities such as dilated vessels, hemorrhages, liver and lung congestion, and brain or heart ischemia. Mutations in two genes, Endoglin (also designated CD105) and ALK-1 (Activin receptor-like kinase 1, also designated TGFβ superfamily RI), are responsible for HHT. Endoglin is mutated in HHT1, and ALK-1 is mutated in HHT2, both of which are thought to be caused by haploinsufficiency. Endoglin and ALK-1 are type III and type I members of the TGFβ receptor superfamily, respectively, that are expressed on vascular endothelial cells. Endoglin can only bind ligands of the TGFβ superfamily via association with the respective ligand binding receptors for TGFβ1, TGFβ3, Activin-A, BMP-2 and BMP-7.
• 产品名称Anti-CD105 antibody [H4-A9]
• 分子量71 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane.
• 其它名称
  • AI528660 antibody
  • AI662476 antibody
  • CD 105 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD105 on HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate using anti-CD105 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining CD105 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-CD105 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-CD105 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of HepG2 cells with CD105 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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