Anti-alpha Actinin antibody
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概述
- 产品描述Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
- 产品名称Anti-alpha Actinin antibody
- 分子量103 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human alpha Actinin aa 350-650.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Plasma membrane. Cytoskeleton.
- 其它名称actinin 1 smooth muscle antibody
Actinin alpha 1 antibody
actinin, alpha 1 antibody
ACTN 1 antibody
Actn1 antibody
ACTN1_HUMAN antibody
Alpha Actinin 1 antibody
Alpha actinin cytoskeletal isoform antibody
Alpha-actinin cytoskeletal isoform antibody
Alpha-actinin-1 antibody
BDPLT15 antibody
F actin cross linking protein antibody
F-actin cross-linking protein antibody
FLJ40884 antibody
FLJ54432 antibody
Non muscle alpha actinin 1 antibody
Non-muscle alpha-actinin-1 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:100
IHC-P: 1:50 -1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Rat colon tissue
Lane 3: Mouse colon tissue
Fig2: ICC staining alpha Actinin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining alpha Actinin in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining alpha Actinin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of MG-63 cells with alpha Actinin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the s
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