Anti-MET antibody [C4-E9]

Anti-MET antibody [C4-E9]

• 概述

• 产品描述The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines. The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
• 产品名称Anti-MET antibody [C4-E9]
• 分子量155 kDa
• 种属反应性Human,Monkey
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Single-pass type I membrane protein.
• 其它名称
  • AUTS9 antibody
  • c met antibody
  • D249 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MET on human MET recombinant protein using anti-MET antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MET on HEK293 (1) and MET-hIgGFc transfected HEK293 (2) cell lysate using anti-MET antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of MET on different cell lysate using anti-MET antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: A549
  Lane 2: COS7
  Lane 3: Hela
  Lane 4: HEK293
  Lane 5: HepG2
  Lane 6: A431

   

  

  Fig4: ICC staining MET (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-MET antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of MCF-7 cells with MET antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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