Anti-C12orf51 antibody [A2-F5-F10]

Anti-C12orf51 antibody [A2-F5-F10]

• 概述

• 产品描述E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
• 产品名称Anti-C12orf51 antibody [A2-F5-F10]
• 分子量438/145/98/262 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Single-pass membrane protein.
• 其它名称
  • C12orf51 antibody
  • HECT domain-containing protein 4 antibody
  • HECTD4 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50
  IHC-P: 1:50-1:100
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.

   

  

  Fig2: ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！