Anti-CD15 antibody [D8-E10]

Anti-CD15 antibody [D8-E10]

• 概述

• 产品描述CD15, also known as Lewis X or LeX, is a carbohydrate antigen that is generated by FucT-IV (alpha 1,3-fucosyltransferase IV). Commonly found on the surface of leukocytes and some tumor cells, CD15 is a trisaccharide that is synthesized when FucT-IV transfers an α-fucose residue onto the β-GlcNAc moiety of cellular N-acetyllactosamines. CD15 functions as an adhesion molecule capable of calcium-mediated homotypic binding. Cells with high surface expression of CD15, therefore, exhibit strong self-aggregation (based on CD15-CD15 interaction) in the presence of calcium. Additionally, CD15 is thought to be a ligand for Selectins (proteins involved in mediating leukocyte-specific cellular interactions), further supporting its role as a cell-adhesion protein.
• 产品名称Anti-CD15 antibody [D8-E10]
• 分子量59 kDa
• 种属反应性Human
• 验证应用ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Golgi Apparatus.
• 其它名称
  • 3-FAL antibody
  • Alpha-3-fucosyltransferase antibody
  • ELAM 1 ligand antibody
  more

• 应用

  ICC: 1:100-1:500
  IHC-P: 1:50-1:200

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  Fig1: ICC staining CD15 (green) and actin filaments (red) in PC-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-CD15 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD15 antibody. Counter stained with hematoxylin.

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