Anti-Bad antibody
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概述
- 产品描述The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes thereby causing restoration of Bax-mediated apoptosis.
- 产品名称Anti-Bad antibody
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Bad N-terminal (KLH-coupled).
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Mitochondrion outer membrane, Cytoplasm
- 其它名称
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Immunocytochemical staining of HepG2 cells using anti-Bad rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-Bad rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of SW480 cells using anti-Bad rabbit polyclonal antibody.
Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Bad rabbit polyclonal antibody.
Fig5: Flow cytometric analysis of MCF-7 cells with Bad antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
