Anti-Glutathione Peroxidase 1 antibody [C5-F6]

Anti-Glutathione Peroxidase 1 antibody [C5-F6]

• 概述

• 产品描述GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
• 产品名称Anti-Glutathione Peroxidase 1 antibody [C5-F6]
• 分子量22 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • AL033363 antibody
  • Cellular glutathione peroxidase antibody
  • Glutathione peroxidase 1 antibody
  more

• 应用

  WB: 1:1,000
  IHC-P: 1:200
  ICC: 1:200
  FC: 1:100-1:200

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  Fig1: Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.
  Positive control:
  Lane 1: THP-1
  Lane 2: HepG2
  Lane 3: 293T

   

  

  Fig2: ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

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