Anti-NGF Recepr antibody [2G2]

Anti-NGF Recepr antibody [2G2]

• 概述

• 产品描述The Trk oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF, suggesting that Trk A participates in the primary signal transduction mechanism of NGF. An additional component of the Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low affinity but is required for efficient signaling. NGFR p75 accelerates Trk A activation and may recruit downstream effector molecules to the liganded complex.
• 产品名称Anti-NGF Recepr antibody [2G2]
• 分子量75kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原Amino acids 393-427 at the C-terminus of NGFR p75 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane
• 其它名称
  • CD271 antibody
  • CD271 antigen antibody
  • Gp80 LNGFR antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500

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  Fig1: Westernblot analysis of NGFR p75 expressionin SK-N-NC whole cell lysate.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

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