Anti-Haptoglobin antibody [5-D3]

Anti-Haptoglobin antibody [5-D3]

• 概述

• 产品描述As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
• 产品名称Anti-Haptoglobin antibody [5-D3]
• 分子量42 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Native protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Extracellular region, secreted.
• 其它名称
  • Binding peptide antibody
  • BP antibody
  • Haptoglobin alpha chain antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Haptoglobin on human lung (1) and human liver (2) tissue lysate using anti-Haptoglobin antibody at 1/500 dilution.

   

  

  Fig2: ICC staining Haptoglobin (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Haptoglobin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Haptoglobin antibody. Counter stained with

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！