Anti-Lamin B1 antibody
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概述
- 产品描述A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear Lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type Lamins, such as Lamin B1, undergo a series of modifications, such as farnesylation and phosphorylation. Lamin B1 is a 586 amino acid protein that is encoded by a gene which, when mutated, is involved in the pathogenesis of autosomal dominant adult-onset leukodystrophy (ADLD), a disease characterized by cerebellar dysfunction and symmetric demyelination of the central nervous system.
- 产品名称Anti-Lamin B1 antibody
- 分子量67 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P
- 抗体类型兔多抗
- 免疫原Amino acids 401-490 mapping near the C-terminus of Lamin B1 of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus inner membrane
- 其它名称
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
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Fig1: Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates. .
Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes (A). Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization (B). .
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